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Produktdetails

Verlag
SPRINGER NATURE
Humana Press Inc.
Erschienen
2004
Sprache
English
Seiten
500
Infos
500 Seiten
237 mm x 163 mm
ISBN
978-1-58829-406-7

Hauptbeschreibung

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Langtext

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Inhaltsverzeichnis

Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection
David O'Neill and Nina Bhardwaj

A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy
Gennady Bocharov, Neville J. Ford, and Burkhard Ludewig

Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres
Ying Waeckerle-Men, Bruno Gander, and Marcus Groettrup

RNA Transfection of Dendritic Cells
Frank Grünebach, Martin R. Müller, and Peter Brossart

Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System
John D. M. Campbell, Christoph Piechaczek, Gregor Winkels, Edith Schwamborn, Daniela Micheli, Sonja Hennemann, and Jürgen Schmitz

Generation of Clinical Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System
Thomas Putz, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer, Georg Bartsch, Lorenz Höltl, and Martin Thurnher

Adenoviral Transduction of Dendritic Cells
Rienk Offringa, Kitty Kwappenberg, Martijn Rabelink, Delphine Rea, and Rob Hoeben

Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy
David O'Neill and Nina Bhardwaj

Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells
I. Jolanda M. de Vries, Gosse J. Adema, Cornelis J. A. Punt, and Carl G. Figdor

Dendritic Cells in Clinical Trials for Multiple Myeloma
Volker L. Reichardt and Peter Brossart

Identification of Tumor-Associated Autoantigens With SEREX
Özlem Türeci, Dirk Usener, Sandra Schneider, and Ugur Sahin

Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy
Petra Weingarten, Petra Lutter, Andreas Wattenberg, Martin Blueggel, Sonja Bailey, Joachim Klose, Helmut E. Meyer, and Christoph Huels

Isolationand Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy
Helga Bernhard, Burkhard Schmidt, Dirk H. Busch, and Christian Peschel

Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers
Norbert Meidenbauer and Andreas Mackensen

Gene Transfer of MHC-Restricted Receptors
Helmut W. H. G. Kessels, Monika C. Wolkers, and Ton N. M. Schumacher

Immunotherapy With CTL Restricted by Nonself MHC
Liquan Gao, Anne-Marie Downs, and Hans J. Stauss

Designing TCR for Cancer Immunotherapy
Ralf-Holger Voss, Jürgen Kuball, Matthias Theobald

Isolation and Expansion of Tumor-Specific CD4+ T-Cells by Means of Cytokine Secretion
Christian Becker

Methods for the Ex Vivo Characterization of Human CD8+ T Subsets Based on Gene Expression and Replicative History Analysis
Nathalie Rufer, Patrick Reichenbach, and Pedro Romero

Regulatory T Cells in Antitumor Therapy: Isolation and Functional Testing of CD4+CD25+ Regulatory T-Cells
Helmut Jonuleit and Edgar Schmitt

Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation
Lucienne Chatenoud

Producing Bispecific and Bifunctional Antibodies
Dipankar Das and Mavanur R. Suresh

Antibody-Cytokine Fusion Proteins for the Therapy of Cancer
Gustavo Helguera and Manuel L. Penichet

Synthesis and Biological Evaluation of a Paclitaxel Immunoconjugate
Ahmad Safavy and Kevin P. Raisch

Cytotoxic Tumor Targeting With scFv Antibody-Modified Liposomes
Cornelia Marty and Reto A. Schwendener

Intravenous Immunoglobin Treatment for Fibrosis, Atherosclerosis, and Malignant Conditions
Ilan Krause and Yehuda Shoenfeld

Study of T-Cell Costimulatory Blockade In Vivo at a Single Cell Level
Minh Diem Vu and Xian Chang Li

Stem Cell Transplantation: Graft-Mediated Antileukemia

Zitat aus einer Besprechung

"...a comprehensive and authoritative collection of currently used protocols for adoptive immunotherapy in both pre-clinical and clinical settings." - Immunology News



"...will be not only useful for basic researchers but also clinical scientists aiming to translate basic research to a clinical setting." - Immunology and Cell Biology

Klappentext

Over the last decade, major advances in our understanding of the immune system and its mechanisms have led to promising new treatment strategies. In Adoptive Immunotherapy: Methods and Protocols, leading experts describe in detail their optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T-cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immonotherapy, and the utilization of regulatory T-cells. Theoretical overviews and discussion of future directions for the improvement of adoptive immunotherapy complete the volume. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Adoptive Immunotherapy: Methods and Protocols offers an authoritative collection of novel techniques to study the adoptive transfer of tailored antigen-specific immune cells and to improve their clinical application.